a simple, fast and commercial method for trypsin purification from bovine pancreas

extraction and acetylation of purified trypsin from bovin pancreas and study of some its physico-chemical properties

آنزیم تریپسین در شرایط قلیایی ناپایدار می باشد .و فعالیت پروتئولیتیکی تریپسین منجربه خود هضمی آن در جایگاههای خاصی می گردد. بنابر این آنزیمی با ناپایداری بالا محسوب میگردد. در سالهای اخیر موفق شدند که با ایجاد تغیرات شیمیایی با اضافه کردن فلزات خاص ، کلسیم و یا عمل استیلاسیون منجر به افزایش پایداری آنزیم تریپسین گردند. مطالعات در حال حاضر نشان می دهد که تریپسین استیله شده فعالیت آنزیمی خود را ...

The Basic Trypsin Inhibitor of Bovine Pancreas

A derivative of the basic pancreatic inhibitor which contained carboxamidomethyl groups on half-cystine residues 14 and 38 was incubated with trypsin and intact inhibitor at pH 5.5. The carboxamidomethyl derivative after separation from trypsin and intact inhibitor released only lysine when incubated with carboxypeptidase B. The derivative was oxidized with performic acid and a peptide correspo...

Simple method for estimating trypsin.

A fast and simple method for modeling of oil swelling in Co2 injection

In this paper, the role of molecular diffusion in mobilization of waterflood residual oil is examined. A moving mesh method is applied to solve the moving interface problem of residual oil blobs swelling by Co2 diffusion through a blocking water phase. The results of this modeling are compared with experimental results of a 2D glass micromodel experiment. Although, the solution method is applie...

A Simple and Fast Method for Field Calibration of Triaxial Gyroscope by using Accelerometer

Isolation from Beef Pancreas of Crystalline Trypsinogen, Trypsin, a Trypsin Inhibitor, and an Inhibitor-trypsin Compound

Methods are described for the isolation and crystallization of trypsinogen, trypsin, a substance which inhibits trypsin, and an inhibitor-trypsin compound. Analyses and some of the properties of these compounds are given.